BioChem - DOS

Repeated fed-batch fermentation using biosensor online control for citric acid production by Yarrowia lipolytica

Journal of biotechnology

Moeller, Lucie; Grü nberg, Mario; Zehnsdorf, Andreas; Aurich, Andreas; Bley, Thomas; Strehlitz, Beate

<P><B>Abstract</B></P><P>Biosensor-controlled substrate feeding was used in a citric acid production process with the yeast strain <I>Yarrowia lipolytica</I> H222 with glucose as the carbon source. The application of an online glucose biosensor measurement facilitated the performance of long-time repeated fed-batch process with automated bioprocess control. Ten cycles of repeated fed-batch fermentation were carried out in order to validate both the stability of the microorganism for citric acid production and the robustness of the glucose biosensor in a long-time experiment. In the course of this fermentation with a duration of 553h, a slight loss of productivity from 1.4g/(L&times;h) to 1.1g/(L&times;h) and of selectivity for citric acid from 91% to 88% was observed. The glucose biosensor provided 6,227 measurements without any loss of activity.</P>

2011

Elsevier

0168-1656

1873-4863

153

3

pp.133-137

10.1016/j.jbiotec.2011.03.013

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART56173325

Citric acid; Yarrowia lipolytica; Glucose biosensor; Biosensor online control; Semicontinuous fermentation; Repeated fed-batch