BioChem - DOS

Biosynthesis of soluble carotenoid holoproteins in Escherichia coli

Scientific reports

de Carbon, Cé line Bourcier; Thurotte, Adrien; Wilson, Adjé lé Perreau, Franç ois; Kirilovsky, Diana

<P>Carotenoids are widely distributed natural pigments that are excellent antioxidants acting in photoprotection. They are typically solubilized in membranes or attached to proteins. In cyanobacteria, the photoactive soluble Orange Carotenoid Protein (OCP) is involved in photoprotective mechanisms as a highly active singlet oxygen and excitation energy quencher. Here we describe a method for producing large amounts of holo-OCP in <I>E.coli</I>. The six different genes involved in the synthesis of holo-OCP were introduced into <I>E. coli</I> using three different plasmids. The choice of promoters and the order of gene induction were important: the induction of genes involved in carotenoid synthesis must precede the induction of the <I>ocp</I> gene in order to obtain holo-OCPs. Active holo-OCPs with primary structures derived from several cyanobacterial strains and containing different carotenoids were isolated. This approach for rapid heterologous synthesis of large quantities of carotenoproteins is a fundamental advance in the production of antioxidants of great interest to the pharmaceutical and cosmetic industries.</P>

2015

Nature Publishing Group

cc-by

2045-2322

5

pp.9085

10.1038/srep09085

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART74295463

https://www.nature.com/articles/srep09085.pdf