초록
<P><B>Abstract</B></P> <P>Response Surface Methodology (RSM) based on Central Composite Design (CCD) was employed to optimize the conditions of enzymatic hydrolysis and fermentation of the seaweed <I>Eucheuma cottonii</I>. The significant influence of cellulose loading, enzyme loading and incubation time in enzymatic hydrolysis that has been screened by Plackett-Burman Design (PBD) was optimized using CCD. The optimum glucose concentration of 24.24 g/L (81% glucose yield) was obtained at 3% (v/v) of cellulose loading, 4% (v/v) of enzyme loading and 54 h incubation time. Subsequently, PBD analysis showed the significant effects of inoculum concentration, pH, temperature and time on fermentation process. Further optimization study by CCD revealed that 12% (v/v) of inoculum concentration, pH 5.2, 32 °C and 72 h of fermentation time enhanced the bioethanol production up to 9.77 g/L with the yield of 0.40 g/g and 78% conversion efficiency. Thus, the RSM based optimization of bioethanol from <I>Eucheuma cottonii</I> showed satisfactory results in this research. In short, it can be concluded that this optimization approach will serve as a good foundation for the realization of a consistent bioethanol production in the future.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Eucheuma cottonii</I> seaweed was employed as the main feedstock for bioethanol production. </LI> <LI> Enzymatic hydrolysis and fermentation were the main conversion technologies utilized. </LI> <LI> Response Surface Methodology was used to optimize the enzymatic hydrolysis and fermentation for bioethanol production. </LI> </UL> </P>