Wheat straw was pretreated using an autohydrolysis process with different temperatures (160-200<SUP>o</SUP>C) and times (10-20min) in order to allow the recovery of hemicellulose in the filtrate and help open up the structure of the biomass for improved accessibility of enzymes during enzymatic hydrolysis. Autohydrolysis at 190<SUP>o</SUP>C for 10min provided the highest overall sugar (12.2/100g raw wheat straw) in the autohydrolysis filtrate and recovered 62.3% of solid residue. Before enzymatic hydrolysis, the pulps obtained from each pretreatment condition were subjected to a refining post-treatment to improve enzyme accessibility. Enzymatic hydrolysis was performed for all the pretreated solids with and without refining post-treatment at the enzyme loadings of 4 and 10FPU/g oven dry substrate for 96h. A total of 30.4g sugars can be recovered from 100g wheat straw at 180<SUP>o</SUP>C for 20min with 4FPU/g enzyme charge.