BioChem - DOS

Efficient Coproduction of Mannanase and Cellulase by the Transformation of a Codon-Optimized Endomannanase Gene from Aspergillus niger into Trichoderma reesei

Journal of agricultural and food chemistry

Sun, Xianhua; Xue, Xianli; Li, Mengzhu; Gao, Fei; Hao, Zhenzhen; Huang, Huoqing; Luo, Huiying; Qin, Lina; Yao, Bin; Su, Xiaoyun

<P>Cellulase and mannanase are both important enzyme additives in animal feeds. Expressing the two enzymes simultaneously within one microbial host could potentially lead to cost reductions in the feeding of animals. For this purpose, we codon-optimized the <I>Aspergillus niger</I> <I>Man5A</I> gene to the codon-usage bias of <I>Trichoderma reesei</I><I>.</I> By comparing the free energies and the local structures of the nucleotide sequences, one optimized sequence was finally selected and transformed into the <I>T. reesei</I> pyridine-auxotrophic strain TU-6. The codon-optimized gene was expressed to a higher level than the original one. Further expressing the codon-optimized gene in a mutated <I>T. reesei</I> strain through fed-batch cultivation resulted in coproduction of cellulase and mannanase up to 1376 U&middot;mL<SUP>&ndash;1</SUP> and 1204 U&middot;mL<SUP>&ndash;1</SUP>, respectively.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2017/jafcau.2017.65.issue-50/acs.jafc.7b05114/production/images/medium/jf-2017-051149_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf7b05114'>ACS Electronic Supporting Info</A></P>

2017

American Chemical Society

0021-8561

1520-5118

65

50

pp.11046-11053

10.1021/acs.jafc.7b05114.s001

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART79259388

https://doi.org/10.1021/acs.jafc.7b05114.s001

mannanase; cellulase; Trichoderma reesei; feed additive