BioChem - DOS

Fungal biofermentation of pine bark producing organic acids and their quantification with capillary electrophoresis

Industrial crops and products

Boke, N.; Siren, H.; Petrik, L.F.

Bioprocessing of pine bark was accomplished using oyster mushroom fungi. The organic acids and inorganic anions in the extracts of fermented samples were identified by capillary electrophoresis (CE). Nine organic acids were simultaneously determined in 12min through the use of an electrolyte composed of 20mM pyrazine-2,3-dicarboxylic acid (2,3-PyDC), 1.5mM CaCl<SUB>2</SUB>.2H<SUB>2</SUB>O and 0.3mM MTAH in methanol:water (10:90, v/v) at pH 9.0. The concurrent determinations of three inorganic anions were performed in less than 10min in an electrolyte consisting of 2.25mM pyromellitic acid, 6.50mM NaOH, 0.75mM hexamethonium hydroxide, and 1.60mM triethanolamine at pH 7.70. The applied analysis methods efficiently separated and identified organic acids and inorganic anions in the extracts. Butyric, maleic and glucuronic acids were the primary organic acids formed via fermentation. Chloride, nitrate and sulphate were detected in all of the samples.

2015

Elsevier

0926-6690

67

pp.41-48

10.1016/j.indcrop.2015.01.020

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART71652123

Pine bark; Oyster mushroom fungi; Batch biofermentation; Capillary electrophoresis; Quantitative analysis