초록
This study was focused on scaling up of 2,3-butanediol (2,3-BD) production, via fermentation in a 150l bioreactor using the newly isolated strain Enterobacter cloacae TERI BD 18. Operational parameters were customized for the strain E. cloacae using a fed- batch strategy, which enhanced the glucose to 2,3-BD conversion yield (0.44g/g). Furthermore, dual stage pH and agitation control regime was considered where in the initial 10h the pH value was 7.5 and the agitation rate used was 200rpm favoring bacterial growth, while these values were subsequently changed to a pH value of 6.5 and an agitation rate of 150rpm leading to enhanced accumulation of 2,3-BD. Production of 85g/l of 2,3-BD was achieved with ethanol and acetoin as the only by-products. Total productivity of 1.73g/l/h with a yield of 0.48g of 2,3-BD per g of glucose was achieved in the 150l bioreactor. Additionally, the total fermentation time was reduced to 50h with this strategy. The integrated approach of fed-batch strategy together with dual regime in pH and agitation control favors 2,3-BD production in the pilot scale bioreactor system employed in this study.