Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli

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논문

Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Bai, B.; Zhou, J.m.; Yang, M.h.; Liu, Y.l.; Xu, X.h.; Xing, J.m.

초록

In this study, microbial production of succinic acid from macroalgae (i.e., Laminaria japonica) was investigated for the first time. The engineered Escherichia coli BS002 exhibited higher molar yield of succinic acid on mannitol (1.39+/-0.01mol/mol) than glucose (1.01+/-0.05mol/mol). After pretreatment and enzymatic hydrolysis, L. japonica hydrolysate was mainly glucose (10.31+/-0.32g/L) and mannitol (10.12+/-0.17g/L), which was used as the substrate for succinic acid fermentation with the recombinant BS002. A final 17.44+/-0.54g/L succinic acid was obtained from the hydrolysate after 72h dual-phase fermentation. The yield was as high as 1.24+/-0.08mol/mol total sugar, which reached 73% of the maximum theoretical yield. The results demonstrate that macroalgae biomass represents a novelty and economical alternative feedstock for biochemicals production.

발행연도

2015

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

185

페이지

pp.56-61

DOI

10.1016/j.biortech.2015.02.081