Metabolic profiling of a Rhizopus oryzae fumaric acid production mutant generated by femtosecond laser irradiation

BioChem - DOS

Search

메타 데이터

바이오화학분류

- 바이오플라스틱
  1. 고무
  2. 플라스틱
- 의료용 화학소재
  1. 치료제

논문

Metabolic profiling of a Rhizopus oryzae fumaric acid production mutant generated by femtosecond laser irradiation

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Yu, S.; Huang, D.; Wen, J.; Li, S.; Chen, Y.; Jia, X.

초록

Femtosecond laser irradiation was employed to induce mutations in Rhizopus oryzae, leading to increases in fumaric acid production. Compared to the parental strain, mutant strain FM19 exhibited an increase in titer and yield of 56.3% and 36.6%, respectively, corresponding to a titer of 49.4g/L and a yield of 0.56g fumaric acid per g glucose. Metabolic profiling by gas chromatography-mass spectrometry revealed that higher levels of carbon (Embden-Meyerhof-Parnas and tricarboxylic acid cycle) and amino acid metabolism were operating in the high-yielding strain; particularly, 4-aminobutyric acid and 5-aminolevulinic acid were increased 10.33- and 7.22-fold, respectively, compared with parental strain during stationary phase. These findings provided new insights into metabolic characterization of high-yielding fumaric acid R. oryzae.

발행연도

2012

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

114

페이지

pp.610-615

DOI

10.1016/j.biortech.2012.03.087