BioChem - DOS

Impact of culture source and linoleic acid (C18:2) on biohydrogen production from glucose under mesophilic conditions

International journal of hydrogen energy

Cata Saady, N.M.; Chaganti, S.R.; Lalman, J.A.; Heath, D.

Genomic and statistical methods were used to demonstrate the effects of linoleic acid (LA) on hydrogen (H<SUB>2</SUB>) production in mixed anaerobic cultures from two sources (designated as A and B). The microbial composition of the control cultures CA and CB were statistically different. Bacteroidaceae (26%) and Clostridiaceae (10%) dominated CA whereas Clostridiaceae (33%) and Bacteroidaceae (10%) dominated CB. Homoacetogens directed 42% of the electron equivalents to acetate production and decreased the H<SUB>2</SUB> yield by 50% in CA compared to CB. The maximum H<SUB>2</SUB> yields (3.11 +/- 0.02 and 3.11 +/- 0.07 mol H<SUB>2</SUB> mol<SUP>-1</SUP> glucose in LA-treated cultures ALA and BLA, respectively) were statistically the same. Cultures ALA and BLA followed the acetate-butyrate pathway while CA and CB followed propionate and homoacetogenic pathways. LA-treated and control cultures were statistically different based on the type and quantity of metabolites; the differences were also confirmed by principal component analysis (PCA).

2012

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

37

5

pp.4036-4045

10.1016/j.ijhydene.2011.11.085

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58487033

Anaerobic; Mixed culture; Principal component analysis (PCA); T-RFLP; Biohydrogen; Linoleic acid (C18:2)