초록
<P><B>Abstract</B></P> <P>A novel carbonyl reductase from <I>Serratia marcescens</I>, <I>Sm</I>CR, was successfully cloned and overexpressed in <I>Escherichia coli</I>. <I>Sm</I>CR could catalyze the asymmetric reduction of long-chain keto acids/esters containing remote carbonyl groups, such as 4-oxo- and 5-oxodecanoic acids, yielding chiral γ- and δ-decalactones with high enantiopurity (up to 99% <I>ee</I>). This is the first report of enzymatic synthesis of (<I>R</I>)-γ- and (<I>R</I>)-δ-decalactones starting from γ-, δ-keto acids using free enzymes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new carbonyl reductase, named <I>Sm</I>CR, was successfully overexpressed in <I>E. coli</I>. </LI> <LI> <I>Sm</I>CR can asymmetrically reduce 4-oxo- and 5-oxodecanoic acids with high enantioselectivity. </LI> <LI> (<I>R</I>)-γ-decalactone in 99% <I>ee</I> and (<I>R</I>)-δ-decalactone in 95% ee with 72–79% yields were obtained. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>