초록
<P>Size exclusion chromatography of β-mannooligosaccharides (β-MOS) mixtures, obtained from ManB-1601 hydrolysis of locust bean gum, resulted in separation of oligosaccharides with various degrees of polymerization (DP 2, 3, and 5). The oligosaccharides were structurally [ESI-MS, FTIR, XRD, TGA, and NMR (<SUP>1</SUP>H and <SUP>13</SUP>C)] and functionally (<I>in vitro</I> fermentation) characterized. DP2 oligosaccharide was composed of two species, (A) mannopyranose β-1,4 mannopyranose and (B) α-1,6-galactosyl-mannopyranose, while DP3 oligosaccharide showed the presence of only one species, i.e. α-<SMALL>d</SMALL>-galactosyl-β-<SMALL>d</SMALL>-mannobiose. ManB-1601 was capable of cleaving near the branch points in the substrate, resulting in oligosaccharides with galactose at the terminal position apart from attacking unsubstituted β-1,4-glycosidic linkages. DP2 and DP3 improved the growth of three out of seven species of <I>Lactobacillus</I> while DP5 resulted in poor growth of all <I>Lactobacillus</I> spp. under <I>in vitro</I> conditions. DP2, DP3, and DP5 were found to inhibit the growth of <I>Escherichia coli, Listeria monocytogenes</I> and <I>Salmonella typhi</I>.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2017/jafcau.2017.65.issue-13/acs.jafc.7b00123/production/images/medium/jf-2017-00123b_0007.gif'></P>