BioChem - DOS

Evaluation of the xylan breakdown potential of eight mesophilic endoxylanases

Enzyme and microbial technology

Cuyvers, S.; Dornez, E.; Moers, K.; Pollet, A.; Delcour, J.A.; Courtin, C.M.

In biomass degradation using simultaneous saccharification and fermentation (SSF), there is a need for efficient biomass degrading enzymes that can work at lower temperatures suitable for yeast fermentation. As xylan is an important lignocellulosic biomass constituent, this study aimed at investigating the possible differences in xylan breakdown potential of endoxylanases using eight different endoxylanases at conditions relevant for SSF. Both solubilising and degrading capacities of the endoxylanases were investigated using water-insoluble and water-soluble oat spelt xylan as model substrates for biomass xylan. Results showed that selecting for combinations of endoxylanases that are efficient at solubilising xylan on the one hand and degrading it to large extent on the other hand, coupled to high specific activities, seems the best option for complete xylan breakdown in lignocellulosic biomass conversion using SSF.

2011

IPC Science and Technology Press ; Elsevier Science Ltd

0141-0229

1879-0909

49

3

pp.305-311

10.1016/j.enzmictec.2011.05.005

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART56946834

[abr] ARX,WS-X; endoxylanase activity towards WS-X based on the formation of reducing xylose; [abr] ARX,WI-X; endoxylanase activity towards WI-X based on the formation of reducing xylose; [abr] ASX,WI-X; endoxylanase solubilising activity on WI-X based on the formation of solubilised xylose; [abr] A/X; arabinose to xylose ratio; [abr] CBM; carbohydrate-binding module; [abr] ES-X; enzyme-solubilised oat spelt; [abr] GH; glycoside hydrolase; [abr] HPAEC-IPAD; high performance anion exchange chromatography with pulsed amperometric detection; [abr] HPSEC; high performance size exclusion chromatography; [abr] MW; molecular weight(s); [abr] RX; reducing xylose; [abr] SSF; simultaneous saccharification and fermentation; [abr] SX; solubilised xylose; [abr] WI-X; water-insoluble oat spelt xylan; [abr] WS-X; water-soluble oat spelt xylan; [abr] XOS; xylo-oligosaccharides; Endoxylanase; Lignocellulosic biomass; Solubilisation; Degradation; Oat;