BioChem - DOS

L-lactic acid production from starch by simultaneous saccharification and fermentation in a genetically engineered Aspergillus oryzae pure culture

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Wakai, S.; Yoshie, T.; Asai-Nakashima, N.; Yamada, R.; Ogino, C.; Tsutsumi, H.; Hata, Y.; Kondo, A.

Lactic acid is a commodity chemical that can be produced biologically. Lactic acid-producing Aspergillus oryzae strains were constructed by genetic engineering. The A. oryzae LDH strain with the bovine l-lactate dehydrogenase gene produced 38g/L of lactate from 100g/L of glucose. Disruption of the wild-type lactate dehydrogenase gene in A. oryzae LDH improved lactate production. The resulting strain A. oryzae LDHΔ871 produced 49g/L of lactate from 100g/L of glucose. Because A. oryzae strains innately secrete amylases, A. oryzae LDHΔ871 produced approximately 30g/L of lactate from various starches, dextrin, or maltose (all at 100g/L). To our knowledge, this is the first report describing the simultaneous saccharification and fermentation of lactate from starch using a pure culture of transgenic A. oryzae. Our results indicate that A. oryzae could be a promising host for the bioproduction of useful compounds such as lactic acid.

2014

Elsevier Applied Science

0960-8524

173

pp.376-383

10.1016/j.biortech.2014.09.094

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70544678

Aspergillus oryzae; l-lactic acid; Starch; Lactate dehydrogenase