BioChem - DOS

Efficient enzymatic synthesis of L-rhamnulose and L-fuculose

Bioorganic & medicinal chemistry letters

Wen, L.; Zang, L.; Huang, K.; Li, S.; Wang, R.; Wang, P.G.

l-Rhamnulose (6-deoxy-l-arabino-2-hexulose) and l-fuculose (6-deoxy-l-lyxo-2-hexulose) were prepared from l-rhamnose and l-fucose by a two-step strategy. In the first reaction step, isomerization of l-rhamnose to l-rhamnulose, or l-fucose to l-fuculose was combined with a targeted phosphorylation reaction catalyzed by l-rhamnulose kinase (RhaB). The by-products (ATP and ADP) were selectively removed by silver nitrate precipitation method. In the second step, the phosphate group was hydrolyzed to produce l-rhamnulose or l-fuculose with purity exceeding 99% in more than 80% yield (gram scale).

2016

Pergamon Press

0960-894x

1464-3405

26

3

pp.969-972

10.1016/j.bmcl.2015.12.051

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART74788750

l-Rhamnulose; l-Fuculose; Enzymatic synthesis; Phosphorylation; De-phosphorylation