초록
<P><B>Abstract</B></P> <P>Synthetic wastewater (SW) at various carbon concentrations (5–60g/l) were evaluated for polyhydroxyalkanoates (PHA) production using the bacteria <I>Pseudomonas pseudoflava</I>. Bacteria showed highest PHA production with 20g/l (57±5%), and highest carbon removal at 5g/l (74±6%) concentrations respectively. Structure, molecular weight, and thermal properties of the produced PHA were evaluated using various analytical techniques. Bacteria produced homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate was used as carbon source; and it produced co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. PHA synthase, the enzyme which produce PHA was extracted from two bacterial strains <I>i.e.</I>, <I>P. pseudoflava</I> and <I>P. palleronii</I> and its molecular weight was analysed using SDS-PAGE. Protein concentration, and PHA synthase enzyme activity of <I>P. pseudoflava</I> and <I>P. palleronii</I> was carried out using spectrophotometer. Results denoted that <I>P. pseudoflava</I> can be used for degradation of organic carbon persistent in wastewaters and their subsequent conversion into PHA.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PHA production from synthetic waste using <I>P. pseudoflava</I> was evaluated. </LI> <LI> High carbon content present in the waste was successfully degraded by bacteria. </LI> <LI> Produced PHA was characterized by NMR, GPC, DSC and TGA. </LI> <LI> PHA synthase enzyme was characterized from <I>P. pseudoflava</I> and <I>P. palleronii</I>. </LI> <LI> PHA production from waste reduces treatment cost & production cost. </LI> </UL> </P>