BioChem - DOS

Engineering the Autotroph Methanococcus maripaludis for Geraniol Production

ACS Synthetic biology

Lyu, Zhe; Jain, Rachit; Smith, Peyton; Fetchko, Travis; Yan, Yajun; Whitman, William B.

<P>The rapid autotrophic growth of the methanogenic archaeon <I><I>Methanococcus maripaludis</I></I> on H<SUB>2</SUB> and CO<SUB>2</SUB> makes it an attractive microbial chassis to inexpensively produce biochemicals. To explore this potential, a synthetic gene encoding geraniol synthase (GES) derived from <I><I>Ocimum basilicum</I></I> was cloned into a <I>M. maripaludis</I> expression vector under selection for puromycin resistance. Recombinant expression of GES in <I>M. maripaludis</I> during autotrophic growth on H<SUB>2</SUB>/CO<SUB>2</SUB> or formate yielded geraniol at 2.8 and 4.0 mg g<SUP>&ndash;1</SUP> of dry weight, respectively. The yield of geraniol decreased 2&ndash;3-fold when organic carbon sources were added to stimulate heterotrophic growth. In the absence of puromycin, geraniol production during autotrophic growth on formate increased to 4.6 mg g<SUP>&ndash;1</SUP> of dry weight. A conceptual model centered on the autotrophic acetyl coenzyme A biosynthetic pathway identified strategies to divert more autotrophic carbon flux to geraniol production.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/asbcd6/2016/asbcd6.2016.5.issue-7/acssynbio.5b00267/production/images/medium/sb-2015-00267p_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/sb5b00267'>ACS Electronic Supporting Info</A></P>

2016

American Chemical Society

cc-by-nc

2161-5063

5

7

pp.577-581

10.1021/acssynbio.5b00267.s001

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75961177

https://figshare.com/articles/journal_contribution/Engineering_the_Autotroph_i_Methanococcus_maripaludis_i_for_Geraniol_Production/2302699/1/files/3940285.pdf

carbon dioxide; hydrogen economy; archaea; methanogen; isoprenoid; synthetic biology