초록
<P><B>Abstract</B></P> <P>In this study, <I>in situ</I> fibrous bed bioreactor (<I>is</I>FBB) was developed at the first time for efficient succinic acid (SA) production by <I>Yarrowia lipolyti</I>ca. After optimization, SA titer, productivity and yield of 51.9g/L, 1.46g/L/h and 0.42g/g were obtained respectively via <I>is</I>FBB fermentation under conditions of 750cm<SUP>2</SUP> cotton towel, 120g/L initial glycerol and 3L/min aeration rate. By fed batch strategy, SA titer raised up to 198.2g/L was achieved, which was the highest value ever reported. In operation stability study, SA productivity showed no obvious decrease after 12 repeated batches of 460h fermentation, and cell viability even recovered within two repeated batches after intentional interruption. This study successfully attained a highly efficient and stable <I>is</I>FBB for enhanced SA production by <I>Y. lipolyti</I>ca.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Efficient SA production by <I>Y. lipolytica</I> via <I>is</I>FBB was developed at the first time. </LI> <LI> Three parameters of <I>is</I>FBB were optimized to achieved SA productivity of 1.46g/L/h. </LI> <LI> Highest titer ever been reported (198.2g/L) was obtained by fed batch strategy. </LI> <LI> Long term repeated fermentation demonstrated <I>is</I>FBB has great operation stability. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>