Srivastava, N.; Singh, J.; Ramteke, P.W.; Mishra, P.K.; Srivastava, M.
초록
Effect of Fe<SUB>3</SUB>O<SUB>4</SUB> nanoparticles (NPs) and Fe<SUB>3</SUB>O<SUB>4</SUB>/Alginate nanocomposites (NCs) have been investigated on production and thermostability of crude cellulase enzyme system obtained by newly isolated thermotolerant Aspergillus fumigatus AA001. Fe<SUB>3</SUB>O<SUB>4</SUB> NPs and Fe<SUB>3</SUB>O<SUB>4</SUB>/Alginate NCs have been synthesized by co-precipitation method and characterized through various techniques. In presence of Fe<SUB>3</SUB>O<SUB>4</SUB> NPs and Fe<SUB>3</SUB>O<SUB>4</SUB>/Alginate NCs, filter paper activity of crude cellulase was increased about 35% and 40%, respectively in 72h as compared to control. Fe<SUB>3</SUB>O<SUB>4</SUB>/Alginate NCs treated crude enzyme was thermally stable up to 8h at 70<SUP>o</SUP>C and retained 56% of its relative activity whereas; control samples could retain only 19%. Further, the hydrolysis of 1.0% alkali treated rice straw using Fe<SUB>3</SUB>O<SUB>4</SUB>/Alginate NCs treated cellulase gave much higher sugar productivity than control at optimal condition. These findings may be utilized in the area of biofuels and biowaste management.