초록
<P>In this study, to obtain higher agmatine yields using the previously developed <I>E. coli</I> strain AUX4 (JM109 Δ<I>speC</I> Δ<I>speF</I> Δ<I>speB</I> Δ<I>argR</I>), the genes encoding glutamate dehydrogenase (<I>gdhA</I>), glutamine synthetase (<I>glnA</I>), phosphoenolpyruvate carboxylase (<I>ppc</I>), aspartate aminotransferase (<I>aspC</I>), transhydrogenase (<I>pntAB</I>), and biosynthetic arginine decarboxylase (<I>speA</I>) were sequentially overexpressed by replacing their native promoters with the heterologous strong <I>trp</I>, core-<I>trc</I>, or 5P<I>tac</I>s promoters to generate the plasmid-free <I>E. coli</I> strain AUX11. The fermentation results obtained using a 3-L bioreactor showed that AUX11 produced 2.93 g L<SUP>-1</SUP> agmatine with the yield of 0.29 g agmatine g<SUP>-1</SUP> glucose in the batch fermentation, and the fed-batch fermentation of AUX11 allowed the production of 40.43 g L<SUP>-1</SUP> agmatine with the productivity of 1.26 g L<SUP>-1</SUP> h<SUP>-1</SUP> agmatine. The results showed that the engineered <I>E. coli</I> strain AUX11 can be used for the industrial fermentative production of agmatine.</P><BR>[FIG OMISSION]</BR>