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Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors

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논문

Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors

학술지

Biochemical engineering journal

저자명

Michelin, M.; de Oliveira Mota, A.M.; Polizeli, M.d.L.T.d.M.; da Silva, D.P.; Vicente, A.A.; Teixeira, J.A.

초록

Oxygenation is an important parameter involved in the design and operation of mixing-sparging bioreactors and it can be analyzed by means of the oxygen mass transfer coefficient (k<SUB>L</SUB>a). In this study, batch fermentations in stirred tank bioreactor (STB) and airlift bioreactor (ALB) were operated under a range of k<SUB>L</SUB>a values, in attempts to optimize and compare the activities of extracellular xylanase and &beta;-xylosidase synthesized by the fungus Aspergillus niger van Tieghem, using corncob as inducer source. The higher xylanase levels were observed on STB (9000Ul<SUP>-1</SUP>; k<SUB>L</SUB>a=30h<SUP>-1</SUP>), while the &beta;-xylosidase production was similar in both bioreactors. However, when the enzymatic activity was compared for the same k<SUB>L</SUB>a value (12h<SUP>-1</SUP>) in STB and ALB, the xylanase and &beta;-xylosidase productions were higher in ALB (7000Ul<SUP>-1</SUP> xylanase; 2100Ul<SUP>-1</SUP> &beta;-xylosidase) than STB (4500Ul<SUP>-1</SUP> xylanase; 1800Ul<SUP>-1</SUP> &beta;-xylosidase).

발행연도

2013

발행기관

Elsevier

ISSN

1369-703x

80

페이지

pp.19-26

주제어

Bioreactors; kLa; Xylanase; β-Xylosidase; Corncob; Aspergillus

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1 2023-12-11

논문; 2013-11-01

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