초록
<P>Recently, the utilization of renewable biomass instead of fossil fuels for producing fuels and chemicals has received much attention due to the global climate change. Among renewable biomass, marine algae are gaining importance as third generation biomass feedstocks owing to their advantages over lignocellulose. Particularly, red macroalgae have higher carbohydrate contents and simpler carbohydrate compositions than other marine algae. In red macroalgal carbohydrates, 3,6-anhydro-<SMALL>L</SMALL>-galactose (AHG) is the main sugar composing agarose along with <SMALL>D</SMALL>-galactose. However, AHG is not a common sugar and is chemically unstable. Thus, not only AHG but also red macroalgal biomass itself cannot be efficiently converted or utilized. Here, we biologically upgraded AHG to a new platform chemical, its sugar alcohol form, 3,6-anhydro-<SMALL>L</SMALL>-galactitol (AHGol), an anhydrohexitol. To accomplish this, we devised an integrated process encompassing a chemical hydrolysis process for producing agarobiose (AB) from agarose and a biological process for converting AB to AHGol using metabolically engineered <I>Saccharomyces cerevisiae</I> to efficiently produce AHGol from agarose with high titers and yields. AHGol was also converted to an intermediate chemical for plastics, isosorbide. To our knowledge, this is the first demonstration of upgrading a red macroalgal biomass component to a platform chemical <I>via</I> a new biological route, by using an engineered microorganism.</P><P>Graphic Abstract</P><P>This study demonstrated the novel biological upgrading (using engineered yeast cells) of 3,6-anhydro-<SMALL>L</SMALL>-galactose, the main but untapped sugar of red macroalgal biomass, to 3,6-anhydro-<SMALL>L</SMALL>-galactitol that can be converted to various valuable chemicals including isosorbide.<BR/><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c9gc04265b'/><BR/></P>