Increased production of succinic acid in Escherichia coli by overexpression of malate dehydrogenase

BioChem - DOS

Search

메타 데이터

바이오화학분류

- 바이오플라스틱
  1. 고무
  2. 플라스틱
- 바이오정밀화학
  1. 용매
  2. 화학제품
- 화장품용 기능성소재
  1. 계면활성제⁄증점제
- 의료용 화학소재
  1. 건강보조식품
  2. 식품첨가제

논문

Increased production of succinic acid in Escherichia coli by overexpression of malate dehydrogenase

학술지

Biotechnology letters. : a monthly journal for the rapid communication of results and developments in all aspects of biotechnology

저자명

Liang, Li-ya; Liu, Rong-ming; Ma, Jiang-feng; Chen, Ke-quan; Jiang, Min; Wei, Ping

초록

<P>Escherichia coli NZN111 is a double mutant with inactivated lactate dehydrogenase and pyruvate formate-lyase. It cannot utilize glucose anaerobically because of its unusually high intracellular NADH/NAD(+) ratio. We have now constructed a recombinant strain, E. coli NZN111/pTrc99a-mdh, which, during anaerobic fermentation, produced 4.3 g succinic acid l(-1) from 13.5 g glucose l(-1). The NADH/NAD(+) ratio decreased from 0.64 to 0.26. Furthermore, dual-phase fermentation (aerobic growth followed by anaerobic phase) resulted in enhanced succinic acid production and reduced byproduct formation. The yield of succinic acid from glucose during the anaerobic phase was 0.72 g g(-1), and the productivity was 1.01 g l(-1) h(-1).</P>

발행연도

2011

발행기관

Springer-Verlag

ISSN

0141-5492

ISSN

1573-6776

권

호

페이지

pp.2439-2444