초록
<P><B>Abstract</B><P>Burkholderia sp. F24, originally isolated from soil, was capable of growth on xylose and removed organic inhibitors present in a hemicellulosic hydrolysate and simultaneously produced poly-3-hydroxybutyrate (P3HB). Using non-detoxified hydrolysate, Burkholderia sp. F24 reached a cell dry weight (CDW) of 6.8 g L−1, containing 48 % of P3HB and exhibited a volumetric productivity (PP3HB) of 0.10 g L−1 h−1. Poly-3-hydroxybutyrate-co-3-hydroxyvalerate copolymers (P3HB-co-3HV) were produced using xylose and levulinic acid (LA) as carbon sources. In shake flask cultures, the 3HV content in the copolymer increased from 9 to 43 mol% by adding LA from 1.0 to 5.0 g L−1. In high cell density cultivation using concentrated hemicellulosic hydrolysate F24 reached 25.04 g L−1 of CDW containing 49 % of P3HB and PP3HB of 0.28 g L−1 h−1. Based on these findings, second-generation ethanol and bioplastics from sugarcane bagasse is proposed.</P></P>