초록
<P><B>Abstract</B></P> <P>Bio-based production of succinic acid (SA) is regarded to supplement the petro-based processing for environmental reasons. However, in order to enhance the industrial competitiveness of bio-based SA, it is necessary to develop a method that is cost-effective, environmental friendly and with high efficiency. In this study, the utilization of fruit and vegetable waste (FVW) hydrolysate for SA production via engineered <I>Yarrowia lipolytica</I> was reported for the first time. In the study, the hydrolysis conditions were firstly optimized and the final glucose concentration of 56.7 g L<SUP>−1</SUP> and a yield of 0.46 g g<SUP>−1</SUP> were obtained with 2% glucoamylase, 1% cellulase, 2% hemicellulase and 0.25% pectinase at pH 5.0 and 55 °C. Then, the feasibility of using the hydrolysate for SA production via <I>Y. lipolytica</I> PSA02004 was demonstrated. SA titer and yield at 43.1 g L<SUP>−1</SUP> and 0.46 g g<SUP>−1</SUP>, respectively were obtained using 100 g L<SUP>−1</SUP> glucose-containing FVW hydrolysate and 4% corn steep liquor (CSL) as fermentation medium by free cell batch fermentation. Finally, <I>in situ</I> fibrous bed bioreactor (<I>is</I>FBB) and fed batch fermentation were demonstrated to be effective approach for improved SA production, from which SA productivity of 0.69 g L<SUP>−1</SUP> h<SUP>−1</SUP> and the resultant SA concentration of 140.6 g L<SUP>−1</SUP> were achieved, respectively. In summary, this study achieved the anticipated target of turning waste into value-added products with high efficiency and cost-effectiveness.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>