초록
<P>Bio-based production of chemicals from renewable resources is becoming increasingly important for sustainable chemical industry. In this study, <I>Escherichia coli</I> was metabolically engineered to produce 1,3-diaminopropane (1,3-DAP), a monomer for engineering plastics. Comparing heterologous C<SUB>4</SUB> and C<SUB>5</SUB> pathways for 1,3-DAP production by genome-scale <I>in silico</I> flux analysis revealed that the C<SUB>4</SUB> pathway employing <I>Acinetobacter baumannii dat</I> and <I>ddc</I> genes, encoding 2-ketoglutarate 4-aminotransferase and L-2,4-diaminobutanoate decarboxylase, respectively, was the more efficient pathway. In a strain that has feedback resistant aspartokinases, the <I>ppc</I> and <I>aspC</I> genes were overexpressed to increase flux towards 1,3-DAP synthesis. Also, studies on 128 synthetic small RNAs applied in gene knock-down revealed that knocking out <I>pfkA</I> increases 1,3-DAP production. Overexpression of <I>ppc</I> and <I>aspC</I> genes in the <I>pfkA</I> deleted strain resulted in production titers of 1.39 and 1.35 g l<SUP>−1</SUP> of 1,3-DAP, respectively. Fed-batch fermentation of the final engineered <I>E. coli</I> strain allowed production of 13 g l<SUP>−1</SUP> of 1,3-DAP in a glucose minimal medium.</P>