Increased fatty acid unsaturation and production of arachidonic acid by homologous over-expression of the mitochondrial malic enzyme in Mortierella alpina

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- 바이오정밀화학
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  2. 화학제품
- 화장품용 기능성소재
  1. 기능성
  2. 계면활성제⁄증점제
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  2. 건강보조식품
  3. 식품첨가제

논문

Increased fatty acid unsaturation and production of arachidonic acid by homologous over-expression of the mitochondrial malic enzyme in Mortierella alpina

학술지

Biotechnology letters. : a monthly journal for the rapid communication of results and developments in all aspects of biotechnology

저자명

Hao, Guangfei; Chen, Haiqin; Du, Kai; Huang, Xiaoyun; Song, Yuanda; Gu, Zhennan; Wang, Lei; Zhang, Hao; Chen, Wei; Chen, Yong Q.

초록

<P>Malic enzyme (ME) catalyses the oxidative decarboxylation of L-malate to pyruvate and provides NADPH for intracellular metabolism, such as fatty acid synthesis. Here, the mitochondrial ME (mME) gene from Mortierella alpina was homologously over-expressed. Compared with controls, fungal arachidonic acid (ARA; 20:4 n-6) content increased by 60?% without affecting the total fatty acid content. Our results suggest that enhancing mME activity may be an effective mean to increase industrial production of ARA in M. alpina.</P>

발행연도

2014

발행기관

Springer-Verlag

ISSN

0141-5492

ISSN

1573-6776

권

호

페이지

pp.1827-1834