BioChem - DOS

Construction of a recombinant Trichoderma reesei strain expressing Aspergillus aculeatus β-glucosidase 1 for efficient biomass conversion

Biotechnology and bioengineering

Nakazawa, Hikaru; Kawai, Tetsushi; Ida, Noriko; Shida, Yosuke; Kobayashi, Yoshinori; Okada, Hirofumi; Tani, Shuji; Sumitani, Jun‐ ichi; Kawaguchi, Takashi; Morikawa, Yasushi; Ogasawara, Wataru

<P><B>Abstract</B></P><P>To develop a <I>Trichoderma reesei</I> strain appropriate for the saccharification of pretreated cellulosic biomass, a recombinant <I>T</I>. <I>reesei</I> strain, X3AB1, was constructed that expressed an <I>Aspergillus aculeatus</I> &beta;&#8208;glucosidase 1 with high specific activity under the control of the <I>xyn3</I> promoter. The culture supernatant from <I>T</I>. <I>reesei</I> X3AB1 grown on 1% Avicel as a carbon source had 63&#8208; and 25&#8208;fold higher &beta;&#8208;glucosidase activity against cellobiose compared to that of the parent strain PC&#8208;3&#8208;7 and that of the <I>T</I>. <I>reesei</I> recombinant strain expressing an endogenous &beta;&#8208;glucosidase I, respectively. Further, the xylanase activity was 30% lower than that of PC&#8208;3&#8208;7 due to the absence of <I>xyn3</I>. X3AB1 grown on 1% Avicel&#8208;0.5% xylan medium produced 2.3&#8208; and 3.3&#8208;fold more xylanase and &beta;&#8208;xylosidase, respectively, than X3AB1 grown on 1% Avicel. The supernatant from X3AB1 grown on Avicel and xylan saccharified NaOH&#8208;pretreated rice straw efficiently at a low enzyme dose, indicating that the strain has good potential for use in cellulosic biomass conversion processes. Biotechnol. Bioeng. 2012;109: 92&ndash;99. &copy; 2011 Wiley Periodicals, Inc.</P>

2012

Wiley Subscription Services, Inc., A Wiley Company

0006-3592

1097-0290

109

1

pp.92-99

10.1002/bit.23296

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58101923

β&#x2010; glucosidase; Trichoderma reesei; xyn3; Aspergillus aculeatus; cellulase; bioconversion;