초록
Hydrogen producing bacteria IODB-O3 was isolated from sludge and identified as Clostridium sp. by 16S rDNA gene analysis. In this study, biohydrogen production process was developed using low-cost agro-waste. Maximum H<SUB>2</SUB> was produced at 37<SUP>o</SUP>C and pH 8.5. Maximum H<SUB>2</SUB> yield was obtained 2.54+/-0.2mol-H<SUB>2</SUB>/mol-reducing sugar from wheat straw pre-hydrolysate (WSPH) and 2.61+/-0.1mol-H<SUB>2</SUB>/mol-reducing sugar from pre-treated wheat straw enzymatic-hydrolysate (WSEH). The cumulative H<SUB>2</SUB> production (ml/L), 3680+/-105 and 3270+/-100, H<SUB>2</SUB> production rate (ml/L/h), 153+/-5 and 136+/-5, and specific H<SUB>2</SUB> production (ml/g/h), 511+/-5 and 681+/-10 with WSPH and WSEH were obtained, respectively. Biomass pre-treatment via steam-explosion generates ample amount of WSPH which remains unutilized for bioethanol production due to non-availability of efficient C<SUB>5</SUB>-fermenting microorganisms. This study shows that Clostridium sp. IODB-O3 is capable of utilizing WSPH efficiently for biohydrogen production. This would lead to reduced economic constrain on the overall cellulosic ethanol process and also establish a sustainable biohydrogen production process.