초록
<P>Trichoderma atroviride 676 was studied to evaluate its efficiency in the production of some lignocellulolytic enzymes, using lignocellulosic residual biomass. Best results were obtained when 3.0?% (w/v) untreated sugarcane bagasse was used (61.3 U mL(-1) for xylanase, 1.9 U mL(-1) for endoglucanase, 0.25 U mL(-1) for FPase, and 0.17 U mL(-1) for β-glucosidase) after 3-4?days fermentation. The maximal enzymatic activity for endoglucanase, FPase, and xylanase were observed at 50-60?C and pH?4.0-5.0, whereas thermal stability at 50?C (CMCase and FPase) or 40?C (xylanase) was obtained after 8?h. Zymograms have shown two bands of 104 and 200?kDa for endoglucanases and three bands for xylanase (23, 36, and 55.7?kDa). The results obtained with T. atroviride strain 676 were comparable to those obtained with the cellulolytic strain Trichoderma reesei RUT-C30, indicating, in the studied conditions, its great potential for biotechnological application, especially lignocellulose biomass hydrolysis.</P>