BioChem - DOS

Maximizing the Efficiency of Multienzyme Process by Stoichiometry Optimization

Chembiochem : a European journal of chemical biology

Dvorak, Pavel; Kurumbang, Nagendra P.; Bendl, Jaroslav; Brezovsky, Jan; Prokop, Zbynek; Damborsky, Jiri

<P><B>Abstract</B></P><P>Multienzyme processes represent an important area of biocatalysis. Their efficiency can be enhanced by optimization of the stoichiometry of the biocatalysts. Here we present a workflow for maximizing the efficiency of a three&#8208;enzyme system catalyzing a five&#8208;step chemical conversion. Kinetic models of pathways with wild&#8208;type or engineered enzymes were built, and the enzyme stoichiometry of each pathway was optimized. Mathematical modeling and one&#8208;pot multienzyme experiments provided detailed insights into pathway dynamics, enabled the selection of a suitable engineered enzyme, and afforded high efficiency while minimizing biocatalyst loadings. Optimizing the stoichiometry in a pathway with an engineered enzyme reduced the total biocatalyst load by an impressive 56&thinsp;%. Our new workflow represents a broadly applicable strategy for optimizing multienzyme processes.</P>

2014

WILEY&#x2010;VCH Verlag

1439-4227

1439-7633

15

13

pp.1891-1895

10.1002/cbic.201402265

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70154311

biocatalysis; biotransformations; kinetic modeling; multienzyme reaction; stoichiometry optimization