초록
<P><B>Highlights</B></P><P>► Assemble the whole MVA pathway from <I>Saccharomyces cerevisiae</I> into the <I>E. coli</I> strain. ► Construct a novel pathway for the isoprene production. ► The strain YJM13 could accumulate isoprene up to 2.48mg/l. ► The strain YJM13 could produce 532mg/l isoprene under the fermentation culture.</P> <P><B>Abstract</B></P><P>In this paper, an original strategy is employed to biosynthesize the isoprene by heterologously co-expressing the <I>Saccharomyces cerevisiae</I> MVA pathway and isoprene synthase (IspS) from <I>Populus alba</I> in the <I>Escherichia coli</I> BL21 (DE3) strain, which was screened from three different IspS enzymes. The finally genetic strain YJM13 harboring the MVA pathway and <I>ispS<SUB>Pa</SUB></I> gene could accumulate isoprene up to 2.48mg/l and 532mg/l under the flask and fed-batch fermentation conditions, respectively, which is about three times and five times to the control strain. The result proves to be higher than that in the report documents. In this way, a potential production system for isoprene from renewable sources via the MVA pathway in <I>E. coli</I> has been provided.</P>