BioChem - DOS

A highly efficient ADH-coupled NADH-recycling system for the asymmetric bioreduction of carbon-carbon double bonds using enoate reductases

Biotechnology and bioengineering

Tauber, Katharina; Hall, Melanie; Kroutil, Wolfgang; Fabian, Walter M.F.; Faber, Kurt; Glueck, Silvia M.

<P><B>Abstract</B></P><P>The asymmetric bioreduction of activated alkenes catalyzed by flavin&#8208;dependent enoate reductases from the OYE&#8208;family represents a powerful method for the production of optically active compounds. For its preparative&#8208;scale application, efficient and economic NADH&#8208;recycling is crucial. A novel enzyme&#8208;coupled NADH&#8208;recycling system is proposed based on the concurrent oxidation of a sacrificial <I>sec</I>&#8208;alcohol catalyzed by an alcohol dehydrogenase (ADH&#8208;A). Due to the highly favorable position of the equilibrium of ene&#8208;reduction versus alcohol&#8208;oxidation, the cosubstrate is only required in slight excess. Biotechnol. Bioeng. 2011; 108:1462&ndash;1467. &copy; 2011 Wiley Periodicals, Inc.</P>

2011

Wiley Subscription Services, Inc., A Wiley Company

0006-3592

1097-0290

108

6

pp.1462-1467

10.1002/bit.23078

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART56794657

https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/bit.23078

cofactor recycling; coupled&#x2010; enzyme approach; alcohol dehydrogenase; asymmetric bioreduction of alkene; enoate reductase;