Systematic approach to assess biohydrogen potential of anaerobic sludge and soil rhizobia as biocatalysts: Influence of crucial factors affecting acidogenic fermentation
메타 데이터
바이오화학분류
바이오정밀화학
연료
논문
Systematic approach to assess biohydrogen potential of anaerobic sludge and soil rhizobia as biocatalysts: Influence of crucial factors affecting acidogenic fermentation
A systematic protocol was designed to enumerate the variation in biohydrogen production with two different biocatalysts (sludge and soil) under different pH and organic loads. Both the biocatalysts showed cumulatively higher H<SUB>2</SUB> production under acidogenic condition (pH 6) than at neutral pH condition. The cumulative hydrogen production was non-linearly fitted with modified Gompertz model and statistically validated. Pretreated soil biocatalyst showed relatively higher H<SUB>2</SUB> production (OLR II, 142+/-5ml) than pretreated sludge (OLR I, 123+/-5ml); which was evidenced by substrate linked dehydrogenase activity and bio-electrochemical analysis. Experimental results revealed agricultural soil as a better biocatalyst than anaerobic sludge for all the operated process conditions. The voltammogram profiles and Tafel slopes revealed dominance of reductive catalytic activity of the pretreated inoculums substantiating dark-fermentation. Soil consortia showed low polarization resistance (2.24kΩ) and high reductive electron transfer efficiency (1.17 Vdec<SUP>-1</SUP>) at a high organic load; thus, rebating high H<SUB>2</SUB> production.