BioChem - DOS

Effect of Dissolved Inorganic Carbon on β-Carotene and Fatty Acid Production in Dunaliella sp

Applied biochemistry and biotechnology

Srinivasan, Ramachandran; Kumar, Velayutham Arumuga; Kumar, Dileep; Ramesh, Nachimuthu; Babu, Subramanian; Gothandam, Kodiveri Muthukalianan

<P>This study aimed to explore the effect of sodium bicarbonate (0-200?mM) on the production of β-carotene and lipid content in Dunaliella salina and Dunaliella bardawil. Total carotenoid and chlorophyll content were determined at regular intervals by a UV-VIS spectrophotometer. The β-carotene and lipid contents were analyzed using high-performance liquid chromatography (HPLC) and gas chromatography coupled with mass spectrometry (GC-MS). The HPLC results revealed a twofold increase of β-carotene in D. salina and D. bardawil cultures grown with sodium bicarbonate. Moreover, total fatty acid profiles from GC-MS indicated a maximum relative percentage of saturated fatty acids (tetradecanoic acid, 10,13-diethyl, methyl ester and methyl 16-methyl-heptadecanoate) compared to polyunsaturated fatty acids in both algae. Our results indicate that the optimum concentration of bicarbonate (100 to 150?mM) was required to stimulate a positive effect on β-carotene production as well as the lipid profile in Dunaliella sp.</P>

2015

Springer-Verlag

0273-2289

1559-0291

175

6

pp.2895-2906

10.1007/s12010-014-1461-6

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART71847960