BioChem - DOS

Bioabatement with hemicellulase supplementation to reduce enzymatic hydrolysis inhibitors

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Cao, G.; Ximenes, E.; Nichols, N.N.; Frazer, S.E.; Kim, D.; Cotta, M.A.; Ladisch, M.

A stepwise removal of inhibitory compounds by bioabatement combined with hemicellulase supplementation was conducted to enhance cellulose hydrolysis of liquid hot water-pretreated corn stover. Results showed that the fungus Coniochaeta ligniaria NRRL30616 eliminated most of the enzyme and fermentation inhibitors from liquid hot water-pretreated corn stover hydrolysates. Moreover, addition of hemicellulases after bioabatement and before enzymatic hydrolysis of cellulose achieved 20% higher glucose yields compared to non-treated samples. This work presents the mechanisms by which supplementation of the fungus with hemicellulase enzymes enables maximal conversion, and confirms the inhibitory effect of xylo-oligosaccharides in corn stover hydrolysates once the dominant inhibitory effect of phenolic compounds is removed.

2015

Elsevier Applied Science

0960-8524

190

pp.412-415

10.1016/j.biortech.2015.04.064

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72187312

Bioabatement; Inhibitors; Hemicellulase supplementation; Enzymatic hydrolysis; Liquid hot water biomass pretreatment