Increased production of FAEEs for biodiesel with lipase enhanced Saccharomyces cerevisiae

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  3. 기타
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논문

Increased production of FAEEs for biodiesel with lipase enhanced Saccharomyces cerevisiae

학술지

Process biochemistry

저자명

Liu, J.F.; Nie, K.L.; Fan, L.H.; Wang, F.; Tan, T.W.; Deng, L.

초록

In this study, we expressed lipase 2 from Candida sp. 99-125 in Saccharomyces cerevisiae, and tried direct biodiesel production. Driven by 3-phosphoglycerate kinase promoter, Lip2 showed high expression level in cytoplasm. SDS-PAGE analysis confirmed the successful lipase expression with a 40kDa molecular weight. The enzyme assay indicated that lipase 2 had a specific activity of 12.12μmol/min/mg toward p-nitrophenyl palmitate. Gas chromatography showed that the main fatty acids of S. cerevisiae lipids were palmitoleic acid (31.79%) and oleic acid (29.84%). By three-step addition of 4% ethanol to culture broth, the yield of fatty acid ethyl esters by recombinant S. cerevisiae reached 11.4mg/g dry cell weight. This work proposed a novel pathway for S. cerevisiae that could be applied for producing biodiesel directly.

발행연도

2013

발행기관

Elsevier Applied Science

ISSN

1359-5113

권

호

페이지

pp.1212-1215

DOI

10.1016/j.procbio.2013.06.003