Monascus ruber as cell factory for lactic acid production at low pH
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
기타
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
치료제
식품첨가제
논문
Monascus ruber as cell factory for lactic acid production at low pH
학술지
Metabolic engineering
저자명
Weusthuis, Ruud A.; Mars, Astrid E.; Springer, Jan; Wolbert, Emil JH; van der Wal, Hetty; de Vrije, Truus G.; Levisson, Mark; Leprince, Audrey; Houweling-Tan, G.Bwee; PHA Moers, Antoine; Hendriks, Sjon NA; Mendes, Odette; Griekspoor, Yvonne; Werten, Marc WT; Schaap, Peter J.; van der Oost, John; Eggink, Gerrit
초록
<P><B>Abstract</B></P> <P>A <I>Monascus ruber</I> strain was isolated that was able to grow on mineral medium at high sugar concentrations and 175g/l lactic acid at pH 2.8. Its genome and transcriptomes were sequenced and annotated. Genes encoding lactate dehydrogenase (LDH) were introduced to accomplish lactic acid production and two genes encoding pyruvate decarboxylase (PDC) were knocked out to subdue ethanol formation. The strain preferred lactic acid to glucose as carbon source, which hampered glucose consumption and therefore also lactic acid production. Lactic acid consumption was stopped by knocking out 4 cytochrome-dependent LDH (CLDH) genes, and evolutionary engineering was used to increase the glucose consumption rate. Application of this strain in a fed-batch fermentation resulted in a maximum lactic acid titer of 190g/l at pH 3.8 and 129g/l at pH 2.8, respectively 1.7 and 2.2 times higher than reported in literature before. Yield and productivity were on par with the best strains described in literature for lactic acid production at low pH.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A <I>Monascus ruber</I> strain was isolated able to grow at 150 g/l lactic acid at pH 2.8. </LI> <LI> NLDH genes were introduced and PDC genes were knocked out to evoke lactic acid production. </LI> <LI> Lactic acid consumption was stopped by knocking out CLDH genes. </LI> <LI> The sugar consumption rate was enhanced three-fold by evolutionary engineering. </LI> <LI> The final strain produced lactic acid to a titer of 190g/l at pH 3.8 and 129g/l at pH 2.8. </LI> </UL> </P>