초록
<P><B>Abstract</B></P> <P>This study evaluated the two hydrolysis strategies, involving one thermal and one dilute acid/enzymatic hydrolysis, to produce high xylose and glucose concentrations from lignocellulose assisted with irradiation pretreatment. Prior to hydrolysis, lignocellulose was pretreated by γ-irradiation at 800KGy. The merits of irradiation pretreatment on lignocellulose were contributed to size-reduced particle distributions and low shear rate of material, which allowed high biomass loadings up to 30–40%(w/v, equals to 23–29wt.%) for the consequent hydrolysis process. Results showed that hemicellulose fraction could achieve ∼84g/L of total sugars containing ∼55g/L xylose and ∼21g/L glucose through this two steps hydrolysis. Cellulose fraction would release ∼251g/L of total sugars consisting of ∼235g/L glucose and ∼16g/L xylose in the ultimate enzymatic hydrolysate. To the best of our knowledge, it was the first report of achieving 235g/L glucose in cellulose enzymatic hydrolysate derived from lignocellulose.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Irradiation pretreatment could reduce particle size and low shear rate of feedstock. </LI> <LI> 251g/L of high sugars content could be achieved from irradiated biomass. </LI> <LI> 30–40%(w/v) of irradiated biomass loadings was employed for the hydrolysis process. </LI> <LI> 235g/L glucose content could be obtained in cellulose enzymatic hydrolysate. </LI> <LI> High sugars concentration from lignocellulose opens up a door to biorefinery. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>