Optimization of photosynthetic hydrogen yield from platinized photosystem I complexes using response surface methodology
메타 데이터
바이오화학분류
바이오정밀화학
연료
논문
Optimization of photosynthetic hydrogen yield from platinized photosystem I complexes using response surface methodology
학술지
International journal of hydrogen energy
저자명
Iwuchukwu, Ifeyinwa J.; Iwuchukwu, Ernest; Le, Rosemary; Paquet, Caitlin; Sawhney, Rapinder; Bruce, Barry; Frymier, Paul
초록
<P><B>Abstract</B></P><P>Light-dependent hydrogen production by platinized Photosystem I isolated from the cyanobacterium <I>Thermosynechococcus elongatus BP-1</I> was optimized using response surface methodology (RSM). The process parameters studied included temperature, light intensity and wavelength, and platinum salt concentration. Application of RSM generated a model that agrees well with the data for H<SUB>2</SUB> yield (<I>R</I><SUP>2</SUP> = 0.99 and <I>p</I> < 0.001). Significant effects on the total H<SUB>2</SUB> yield were seen when the platinum salt concentration and temperature were varied during platinization. However, light intensity during platinization had a minimal effect on the total H<SUB>2</SUB> yield within the region studied. The values of the parameters used during the platinization that optimized the production of H<SUB>2</SUB> were light intensity of 240 μE m<SUP>−2</SUP> s<SUP>−1</SUP>, platinum salt concentration of 636 μM and temperature of 31 °C. A subsequent validation experiment at the predicted conditions for optimal process yield gave the maximum H<SUB>2</SUB> yield measured in the study, which was 8.02 μmol H<SUB>2</SUB> per mg chlorophyll.</P> <P><B>Highlights</B></P><P>► We used RSM to optimize photosynthetic hydrogen yield from platinized Photosystem I complexes. ► We examined the effect of process variables on the rate of hydrogen production. ► Platinum concentration and temperature had a significant effect on H<SUB>2</SUB> yield. ► Data from the predicted optimum conditions validated the model predictions.</P>