초록
<P><B>Abstract</B></P> <P>This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion. The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g<SUP>−1</SUP> VS (volatile solids) and 0.090, 0.149, and 0.227 d<SUP>−1</SUP> for SB, SP, and LV, respectively. The chemical composition and the crystalline structure of the substrates as well as their changes during the anaerobic digestion were inspected, and their impacts on the characteristics of methane production were assessed. The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable. The methane production has limit correlation with the crystalline structure of the substrates. Microbial community structure was analyzed to elucidate functional microorganism contributing to methane production of different substrate. The abundance of <I>Bacteroidetes</I> and <I>Firmicutes</I> was most affected by the substrate, and positively related to methane yields.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The methane yields and hydrolysis kinetic constants of SB, SP, and LV were evaluated. </LI> <LI> The chemical and physical changes of the substrates during digestion were inspected. </LI> <LI> Correlation was established between chemical changes and methane production. </LI> <LI> The crystalline structure of cellulose had limit effect to anaerobic digestion of SB. </LI> <LI> The abundance of <I>Bacteroidetes</I> and <I>Firmicutes</I> was positively related to methane yields. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>