The performance of Saccharomyces cerevisiae MBG3964, a strain able to tolerate >18% v/v ethanol, was compared to leading industrial ethanol strain, Fermentis Ethanol Red, under high gravity corn mash fermentation conditions. Compared to the industrial ethanol strain, MBG3964 gave increased alcohol yield (140gL<SUP>-1</SUP> vs. 126gL<SUP>-1</SUP>), lower residual sugar (4gL<SUP>-1</SUP> vs. 32gL<SUP>-1</SUP>), and lower glycerol (11gL<SUP>-1</SUP> vs. 12gL<SUP>-1</SUP>). After 72h fermentation, MBG3964 showed about 40% viability, whereas the control yeast was only about 3% viable. Based on modelling, the higher ethanol tolerant yeast could increase the profitability of a corn-ethanol plant and help it remain viable through higher production, lower unit heating requirements and extra throughput. A typical 50Mgaly<SUP>-1</SUP> dry mill ethanol plant that sells dried distiller's grain could potentially increase its profit by nearly $US3.4My<SUP>-1</SUP> due solely to the extra yield, and potentially another $US4.1My<SUP>-1</SUP> if extra throughput is possible.