D-lactic acid production from xylose in engineered Escherichia coli SZ470

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논문

D-lactic acid production from xylose in engineered Escherichia coli SZ470

학술지

Advanced materials research : AMR

저자명

Zheng, Zhao Min; Tian; Wang, Jin Hua; Wang, Yong Ze; Zhou, Sheng De

초록

WD100, knocked out adhE of Escherichia coli SZ470 and inserted ldhA into Escherichia coli WD01, was genetically engineered to utilize xylose. D-lactate production was investigated for shake flask cultures with xylose. In 64h WD100 produce 10.1g/L D-lactate in the shaking flask And it consumed 25g/L xylose during the ending of fermentation.This volumetric productivity with xylose is 0.14 g·L-1·h-1.Because of pyruvate decarboxylase (poxB) expressed in flask fermention,acetate production was up to 4.7g/L.Succinate,formate,ethanol was also produced as a minor product during fermentation.

발행연도

2013

발행기관

Trans Tech Publications, Ltd.

ISSN

1022-6680

ISSN

1662-8985

권

641

페이지

pp.721-724