Metabolic evolution and 13C flux analysis of a succinate dehydrogenase deficient strain of Yarrowia lipolytica

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논문

Metabolic evolution and 13C flux analysis of a succinate dehydrogenase deficient strain of Yarrowia lipolytica

학술지

Biotechnology and bioengineering

저자명

Yuzbashev, Tigran V.; Bondarenko, Pavel Yu.; Sobolevskaya, Tatiana I.; Yuzbasheva, Evgeniya Yu.; Laptev, Ivan A.; Kachala, Vadim V.; Fedorov, Alexander S.; Vybornaya, Tatiana V.; Larina, Anna S.; Sineoky, Sergey P.

초록

ABSTRACTBio‐based succinic acid production can redirect industrial chemistry processes from using limited hydrocarbons to renewable carbohydrates. A fermentation process that does not require pH‐titrating agents will be advantageous to the industry. Previously, a Yarrowia lipolytica strain that was defective for succinate dehydrogenase was constructed and was found to accumulate up to 17.5 g L−1 of succinic acid when grown on glycerol without buffering. Here, a derivative mutant was isolated that produced 40.5 g L−1 of succinic acid in 36 h with a yield of 0.32 g g−1 glycerol. A combination approach of induced mutagenesis and metabolic evolution allowed isolation of another derivative that could utilize glucose efficiently and accumulated 50.2 g L−1 succinic acid in 54 h with a yield of 0.43 g g−1. The parent strain of these isolated mutants was used for [1,6‐13C2]glucose assimilation analysis. At least 35% glucose was estimated to be utilized through the pentose phosphate pathway, while ≥84% succinic acid was formed through the oxidative branch of the tricarboxylic acid cycle. Biotechnol. Bioeng. 2016;113: 2425–2432. © 2016 Wiley Periodicals, Inc.

발행연도

2016

ISSN

0006-3592

ISSN

1097-0290

권

113

호

페이지

pp.2425-2432

DOI