초록
<P><B>Abstract</B></P> <P> <I>In situ</I> fibrous bed bioreactor (<I>is</I>FBB) for efficient succinic acid (SA) production by <I>Yarrowia lipolytica</I> was firstly developed in our former study. In this study, agricultural residues including wheat straw, corn stalk and sugarcane bagasse were investigated for the improvement of <I>is</I>FBB, and sugarcane bagasse was demonstrated to be the best immobilization material. With crude glycerol as the sole carbon source, optimization for <I>is</I>FBB batch fermentation was carried out. Under the optimal conditions of 20g sugarcane bagasse as immobilization material, 120gL<SUP>−1</SUP> crude glycerol as carbon source and 4Lmin<SUP>−1</SUP> of aeration rate, the resultant SA concentration was 53.6gL<SUP>−1</SUP> with an average productivity of 1.45gL<SUP>−1</SUP> h<SUP>−1</SUP> and a SA yield of 0.45gg<SUP>−1</SUP>. By feeding crude glycerol, SA titer up to 209.7gL<SUP>−1</SUP> was obtained from fed batch fermentation, which was the highest value that ever reported.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel <I>in situ</I> fibrous bed bioreactor was constructed for efficient SA production. </LI> <LI> Crude glycerol was utilised as carbon source in fermentation by <I>Yarrowia lipolytica</I>. </LI> <LI> The highest SA titer of 209.7gL<SUP>−1</SUP> was achieved using naturally derived feedstock. </LI> <LI> Reuse of fermented by-products as insect feed and compost were demonstrated. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>