BioChem - DOS

Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Jin, Mingjie; Bothfeld, William; Austin, Samantha; Sato, Trey K.; La Reau, Alex; Li, Haibo; Foston, Marcus; Gunawan, Christa; LeDuc, Richard D.; Quensen, John F.; Mcgee, Mick; Uppugundla, Nirmal; Higbee, Alan; Ranatunga, Ruwan; Donald, Charles W.; Bone, Gwen; Ragauskas, Arthur J.; Tiedje, James M.; Noguera, Daniel R.; Dale, Bruce E.; Zhang, Yaoping; Balan, Venkatesh

<P><B>Abstract</B></P> <P>To minimize the change of lignocellulosic hydrolysate composition during storage, the effects of storage conditions (temperature, pH and time) on the composition and fermentability of hydrolysate prepared from AFEX&trade; (Ammonia Fiber Expansion &ndash; a trademark of MBI, Lansing, MI) pretreated corn stover were investigated. Precipitates formed during hydrolysate storage increased with increasing storage pH and time. The precipitate amount was the least when hydrolysate was stored at 4&deg;C and pH 4.8, accounting for only 0.02% of the total hydrolysate weight after 3-month storage. No significant changes of NMR (Nuclear Magnetic Resonance) spectra and concentrations of sugars, minerals and heavy metals were observed after storage under this condition. When pH was adjusted higher before fermentation, precipitates also formed, consisting of mostly struvite (MgNH<SUB>4</SUB>PO<SUB>4</SUB>&middot;6H<SUB>2</SUB>O) and brushite (CaHPO<SUB>4</SUB>&middot;2H<SUB>2</SUB>O). <I>Escherichia coli</I> and <I>Saccharomyces cerevisiae</I> fermentation studies and yeast cell growth assays showed no significant difference in fermentability between fresh hydrolysate and stored hydrolysate.</P> <P><B>Highlights</B></P> <P> <UL> <LI> AFEX hydrolysate storage temperature, pH and time were investigated. </LI> <LI> Hydrolysate composition and fermentability before and after storage were examined. </LI> <LI> Precipitates formed during storage increased with increasing pH and time. </LI> <LI> Hydrolysate stored at 4&deg;C and pH 4.8 formed little precipitate. </LI> <LI> Little change of composition and fermentability was found after storage. </LI> </UL> </P>

2013

Elsevier

0960-8524

147

pp.212-220

10.1016/j.biortech.2013.08.018

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART66970398

Hydrolysate storage; Hydrolysate characterization; AFEX; Hydrolysate fermentation; Hydrolysate precipitate