초록
<P><B>Abstract</B></P> <P>1,3-Propanediol (1,3-PD), a valuable bulk industrial material, has attracted increasing interest in recent years. A novel strain GYL isolated from soil samples could efficiently convert glycerol to 1,3-PD anaerobically. The physiological and biochemical characteristics of strain GYL were determined, indicating that strain GYL is a member of <I>Clostridium perfringens</I> with the neighbor-joining method of 16S rRNA gene sequences. The fermentation properties of strain GYL were also investigated systematically, which showed that the strain has a fast growth speed and high tolerance to 200g/L glycerol. Batch fermentation was carried out at a high glycerol concentration of 100g/L, and strain GYL produced 36.7g/L 1,3-PD. In fed-batch fermentation, strain GYL showed a maximum productivity of 2.0g/(L·h), and produced 40.0g/L 1,3-PD, with a high yield of 0.68mol 1,3-PD/mol glycerol. This study shows that the newly isolated strain GYL may have potential for 1,3-PD production from glycerol.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new 1,3-propanediol (1,3-PD) producer <I>Clostridium perfringens</I> GYL was isolated. </LI> <LI> Strain GYL has a fast growth speed and high productivity of 1,3-PD (2.0g/(L·h)). </LI> <LI> Strain GYL could tolerate high concentrations of glycerol up to 200g/L. </LI> </UL> </P>