초록
<P><B>Abstract</B></P> <P>An efficient scheme was developed for the conversion of wheat straw (WS) into bioethanol, silica and lignin. WS was pre-extracted with 0.2 mol/L sodium hydroxide at 30 °C for 5 h to remove about 91% of initial silica. Subsequently, the alkaline-pretreated solids were subjected to alkaline hydrogen peroxide (AHP) pretreatment with 40 mg hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>)/g biomass at 50 °C for 7 h to prepare highly digestible substrate. The results of enzymatic hydrolysis demonstrated that the sequential alkaline-AHP pretreated WS was efficiently hydrolyzed at 10% (w/v) solids loading using an enzyme dosage of 10 mg protein/g glucan. The total sugar conversion of 92.4% was achieved. Simultaneous saccharification and co-fermentation (SSCF) was applied to produce ethanol from the two-stage pretreated substrate using <I>Saccharomyces cerevisiae</I> SR8u strain. Ethanol with concentration of 31.1 g/L was produced. Through the proposed process, about 86.4% and 54.1% of the initial silica and lignin were recovered, respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sequential NaOH extraction-AHP treatment efficiently fractionated wheat straw. </LI> <LI> The proposed process produced ethanol and recovers lignin and silica. </LI> <LI> Alkaline pre-extraction substantially reduced H<SUB>2</SUB>O<SUB>2</SUB> demand during AHP treatment. </LI> <LI> Conventional SSCF process for ethanol production from wheat straw was investigated. </LI> <LI> High titer ethanol was produced with relatively low enzyme loadings. </LI> </UL> </P>