Improving co-fermentation of glucose and xylose by adaptive evolution of engineering xylose-fermenting Saccharomyces cerevisiae and different fermentation strategies
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Improving co-fermentation of glucose and xylose by adaptive evolution of engineering xylose-fermenting Saccharomyces cerevisiae and different fermentation strategies
<P><B>Abstract</B></P> <P>Xylose utilization of engineered yeast is vulnerable to inhibitors generated during pretreatment of lignocellulose. In this study, adaptive evolution was applied to enhance the tolerance of xylose-fermenting strain. Compared to the parental strain, the ethanol yield was increased by 60% and 80% for the adapted strain (E7-403) when xylose was used as the sole carbon resource with 20% and 50% inhibitor cocktails, respectively. E7-403 removed furfural more effectively than parental strain (E7) in the fermentation with 100% inhibitor cocktails. In the fermentation with mixed sugar and high inhibitor concentration, glucose was depleted within 36 h for E7-403 while 6.1 g/L glucose was still left after 120 h for E7. Consequently, ethanol yield of E7-403 was 22.9% higher than that of E7. It was demonstrated that E7-403 strain exhibited an enhanced ability for regulating cellular reactive oxygen species, which alleviated the harmful effects of inhibitors. Meanwhile, E7-403 strain was further applied in co-culture and pre-fermentation process to improve xylose utilization.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Tolerance to multiple inhibitors was enhanced by adaptive evolution. </LI> <LI> Adapted strain converted furfural more efficiently than parental strain. </LI> <LI> Adapted strain downregulated the accumulation of ROS with inhibitors. </LI> <LI> Xylose utilization were further improved by co-culture and pre-fermentation process. </LI> </UL> </P>