초록
<P>As novel low-viscosity and environmentally-friendly reaction media, deep eutectic solvents (DESs) have gained much attraction in biocatalysis. In this study, various DESs were prepared and their effects on the asymmetric oxidation of racemic 1-(4-methoxyphenyl)ethanol (MOPE) catalyzed by <I>Acetobacter</I> sp. CCTCC M209061 cells to give enantiopure (<I>S</I>)-MOPE were examined. The best results were obtained with the DES [ChCl][Gly] and its concentration exerted a significant influence on the reaction, with the optimal content being 10% (v/v). In the [ChCl][Gly]-containing system, the substrate concentration was substantially increased (55 mmol L<SUP>−1</SUP><I>vs.</I> 30 mmol L<SUP>−1</SUP>) as compared with the [ChCl][Gly]-free aqueous system, while the residual substrate <I>e.e.</I> was kept as high as 99.9%. The good biocompatibility of [ChCl][Gly] with the cells and the improved cell membrane permeability in the [ChCl][Gly]-containing system could partly account for the clearly enhanced reaction efficiency.</P>